1x first strand buffer (Thermo Fisher)
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Thermo Fisher
1x first strand buffer
1x First Strand Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/first+strand+buffer/Tris+EDTA+buffer%2C+for+molecular+biology%2C+DNAse%2C+RNAse%2C+Protease+free+ready+to+use%2C+pH+8%2E0/pm42002808-95-17-21
Average 95 stars, based on 1 article reviews
1x First Strand Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/first+strand+buffer/Tris+EDTA+buffer%2C+for+molecular+biology%2C+DNAse%2C+RNAse%2C+Protease+free+ready+to+use%2C+pH+8%2E0/pm42002808-95-17-21
Average 95 stars, based on 1 article reviews
1x first strand buffer - by Bioz Stars,
2026-09
95/100 stars
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Reverse Transcription:Article Title: CD13 is a bona-fide marker of bovine pre-adipocytes with potential in cultivated fat applications. Article Snippet: RNA (300 ng) was incubated with 0.5 μl random primers (250ng/μl, Promega, 1181), 1 μl dNTPs (10mM, Invitrogen, 18427- 013) and nuclease free-water in a thermocycler (Biometra) at 65 °C for 5 min, followed by 5 min at 4 °C. .. Then, 1 μl DTT (0.1 M, Invitrogen, 18080-93), 1 μl RNasin Plus RNase inhibitor (40 U/μL, Promega, N2611), 1 μl SuperScript III reverse transcriptase (Invitrogen, 18080-93), and 4 μl Article Title: cxcl18b -defined transitional state-specific nitric oxide drives injury-induced Müller glia cell-cycle re-entry in the zebrafish retina Article Snippet: .. Interesting cells were collected into a 1.5 ml tube containing lysis buffer (20 mg/ml PK in TE buffer) and total RNA was isolated using this lysis buffer. cDNA synthesis was conducted by adding RT mix containing: 200 U Superscript II reverse transcriptase (Invitrogen, 18064‐014), 1 × Article Title: Impaired function of Vγ9Vδ2 T cells in frail elderly. Article Snippet: .. The RT reaction mixture contained 0.5 μL SuperScript II reverse transcriptase (200 U/μL, Invitrogen), 0.25 μL RNase inhibitor (40 U/μL, Clontech), 2 μL 5× SuperScript II Article Title: Compositions, devices, systems, and methods for using a nanopore Article Snippet: Five micrograms of mRNA is mixed with 1 mg random primer (Life Technologies), incubated at 70° C. for 10 minutes, and lyophilized. .. The lyophilized sample is resuspended in 50 ml of 1× Article Title: Pharmacological perturbation of splicing elicits SMG1 reduction: Implications for cancer therapy Article Snippet: After RNA quantification using NanoDrop, 4.000 μg of RNA were used for cDNA generation, by mixing 4.000 μg in 13 μL RNasa free water with 1 μL of GeneAmp dNTP Blend 100 mM (Applied Biosystems) and 1 μL of Random Hexamer (Invitrogen). .. The mix was incubated for 5 minutes in 65°C, and, after that, 1 μL of RNase OUT Recombinant Ribonuclease Inhibitor, 1 μL of M-MLV Reverse Transcriptase, 1 μL of 0.1M DTT, and 4 μL 5× Article Title: A cocktail vaccine with monkeypox virus antigens confers protection without selecting mutations in potential immune evasion genes in the vaccinia WR strain challenge. Article Snippet: Total RNA was reverse transcribed with random nonamer primers (Sigma) as previously established (61, 65). .. The 20 μL cDNA synthesis system contained the following: 10 μL RNA template, 4 μL 5× Incubation:Article Title: CD13 is a bona-fide marker of bovine pre-adipocytes with potential in cultivated fat applications. Article Snippet: RNA (300 ng) was incubated with 0.5 μl random primers (250ng/μl, Promega, 1181), 1 μl dNTPs (10mM, Invitrogen, 18427- 013) and nuclease free-water in a thermocycler (Biometra) at 65 °C for 5 min, followed by 5 min at 4 °C. .. Then, 1 μl DTT (0.1 M, Invitrogen, 18080-93), 1 μl RNasin Plus RNase inhibitor (40 U/μL, Promega, N2611), 1 μl SuperScript III reverse transcriptase (Invitrogen, 18080-93), and 4 μl Article Title: Compositions, devices, systems, and methods for using a nanopore Article Snippet: Five micrograms of mRNA is mixed with 1 mg random primer (Life Technologies), incubated at 70° C. for 10 minutes, and lyophilized. .. The lyophilized sample is resuspended in 50 ml of 1× Article Title: Pharmacological perturbation of splicing elicits SMG1 reduction: Implications for cancer therapy Article Snippet: After RNA quantification using NanoDrop, 4.000 μg of RNA were used for cDNA generation, by mixing 4.000 μg in 13 μL RNasa free water with 1 μL of GeneAmp dNTP Blend 100 mM (Applied Biosystems) and 1 μL of Random Hexamer (Invitrogen). .. The mix was incubated for 5 minutes in 65°C, and, after that, 1 μL of RNase OUT Recombinant Ribonuclease Inhibitor, 1 μL of M-MLV Reverse Transcriptase, 1 μL of 0.1M DTT, and 4 μL 5× Amplification:Article Title: Nucleotide analogues Article Snippet: “Phospho” This lane was loaded with the amplification product obtained from amplifying genomic RNA from the 2019 Novel Coronavirus; Strain: 2019-nCoV/USA-WA1/2020 (Reference: ATCC-VR-1986D, ATCC), using the RdRp catalytic domain of RNA-directed RNA polymerase (Reference: PX-COV-P006, ProteoGenix) of SARS-CoV2 and the RNA primers (same as the lane “RNA Primer”), using the 10× First Strand Buffer and RNase Inhibitor from Amino Allyl MessageAmpTM II aRNA Amplification Kit (Reference: AM1753, Thermo), and rNTPs in equimolar amounts, except that the standard amount for rATP was split 50:50 between rATP and Compound C. “Fluoro” This lane was loaded with the amplification product obtained from amplifying genomic RNA from the 2019 Novel Coronavirus; Strain: 2019-nCoV/USA-WA1/2020 (Reference: ATCC-VR-1986D, ATCC), using the RdRp catalytic domain of RNA-directed RNA polymerase (Reference: PX-COV-P006, ProteoGenix) of SARS-CoV2 and the RNA primers (same as the lane “RNA Primer”), using the 10× First Strand Buffer and RNase Inhibitor from Amino Allyl MessageAmpTM II aRNA Amplification Kit (Reference: AM1753, Thermo), and rNTPs in equimolar amounts, except that the standard amount for rATP was split 50:50 between rATP and Compound B. .. “Hydroxy” This lane was loaded with the amplification product obtained from amplifying genomic RNA from the 2019 Novel Coronavirus; Strain: 2019-nCoV/USA-WA1/2020 (Reference: ATCC-VR-1986D, ATCC), using the RdRp catalytic domain of RNA-directed RNA polymerase (Reference: PX-COV-P006, ProteoGenix) of SARS-CoV2 and the RNA primers (same as the lane “RNA Primer”), using the 10× Lysis:Article Title: cxcl18b -defined transitional state-specific nitric oxide drives injury-induced Müller glia cell-cycle re-entry in the zebrafish retina Article Snippet: .. Interesting cells were collected into a 1.5 ml tube containing lysis buffer (20 mg/ml PK in TE buffer) and total RNA was isolated using this lysis buffer. cDNA synthesis was conducted by adding RT mix containing: 200 U Superscript II reverse transcriptase (Invitrogen, 18064‐014), 1 × Isolation:Article Title: cxcl18b -defined transitional state-specific nitric oxide drives injury-induced Müller glia cell-cycle re-entry in the zebrafish retina Article Snippet: .. Interesting cells were collected into a 1.5 ml tube containing lysis buffer (20 mg/ml PK in TE buffer) and total RNA was isolated using this lysis buffer. cDNA synthesis was conducted by adding RT mix containing: 200 U Superscript II reverse transcriptase (Invitrogen, 18064‐014), 1 × cDNA Synthesis:Article Title: cxcl18b -defined transitional state-specific nitric oxide drives injury-induced Müller glia cell-cycle re-entry in the zebrafish retina Article Snippet: .. Interesting cells were collected into a 1.5 ml tube containing lysis buffer (20 mg/ml PK in TE buffer) and total RNA was isolated using this lysis buffer. cDNA synthesis was conducted by adding RT mix containing: 200 U Superscript II reverse transcriptase (Invitrogen, 18064‐014), 1 × Article Title: IL-4/STAT6-signaling Influences Local Inflammation and Regeneration Processes During Acute Pancreatitis and Promotes Fibrosis by a Direct Activation of Pancreatic Fibroblasts During Chronic Pancreatitis. Article Snippet: Total RNA was extracted from macrophages, fibroblasts as well as pancreatic tissue using Trizol reagent (15596026, life technologies) according to the manufacturer’s instructions. .. The RNA samples with an A260 /A280 ratio between 1.5 and 2.0 were used for the following cDNA synthesis reaction: 2 μg RNA; 5 μM OligodT primers; 75 ng random primers; 0.5 μM dNTP Mix; 1 × Article Title: Compositions, devices, systems, and methods for using a nanopore Article Snippet: Five micrograms of mRNA is mixed with 1 mg random primer (Life Technologies), incubated at 70° C. for 10 minutes, and lyophilized. .. The lyophilized sample is resuspended in 50 ml of 1× Article Title: A cocktail vaccine with monkeypox virus antigens confers protection without selecting mutations in potential immune evasion genes in the vaccinia WR strain challenge. Article Snippet: Total RNA was reverse transcribed with random nonamer primers (Sigma) as previously established (61, 65). .. The 20 μL cDNA synthesis system contained the following: 10 μL RNA template, 4 μL 5× Recombinant:Article Title: cxcl18b -defined transitional state-specific nitric oxide drives injury-induced Müller glia cell-cycle re-entry in the zebrafish retina Article Snippet: .. Interesting cells were collected into a 1.5 ml tube containing lysis buffer (20 mg/ml PK in TE buffer) and total RNA was isolated using this lysis buffer. cDNA synthesis was conducted by adding RT mix containing: 200 U Superscript II reverse transcriptase (Invitrogen, 18064‐014), 1 × Article Title: Pharmacological perturbation of splicing elicits SMG1 reduction: Implications for cancer therapy Article Snippet: After RNA quantification using NanoDrop, 4.000 μg of RNA were used for cDNA generation, by mixing 4.000 μg in 13 μL RNasa free water with 1 μL of GeneAmp dNTP Blend 100 mM (Applied Biosystems) and 1 μL of Random Hexamer (Invitrogen). .. The mix was incubated for 5 minutes in 65°C, and, after that, 1 μL of RNase OUT Recombinant Ribonuclease Inhibitor, 1 μL of M-MLV Reverse Transcriptase, 1 μL of 0.1M DTT, and 4 μL 5× Concentration Assay:Article Title: Impaired function of Vγ9Vδ2 T cells in frail elderly. Article Snippet: .. The RT reaction mixture contained 0.5 μL SuperScript II reverse transcriptase (200 U/μL, Invitrogen), 0.25 μL RNase inhibitor (40 U/μL, Clontech), 2 μL 5× SuperScript II Polymerase Chain Reaction:Article Title: Pharmacological perturbation of splicing elicits SMG1 reduction: Implications for cancer therapy Article Snippet: After RNA quantification using NanoDrop, 4.000 μg of RNA were used for cDNA generation, by mixing 4.000 μg in 13 μL RNasa free water with 1 μL of GeneAmp dNTP Blend 100 mM (Applied Biosystems) and 1 μL of Random Hexamer (Invitrogen). .. The mix was incubated for 5 minutes in 65°C, and, after that, 1 μL of RNase OUT Recombinant Ribonuclease Inhibitor, 1 μL of M-MLV Reverse Transcriptase, 1 μL of 0.1M DTT, and 4 μL 5× |
