Review



5× first strand buffer  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Thermo Fisher 5× first strand buffer
    RT mix-II
    5× First Strand Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5× first strand buffer/product/Thermo Fisher
    Average 96 stars, based on 1 article reviews
    5× first strand buffer - by Bioz Stars, 2026-05
    96/100 stars

    Images

    1) Product Images from "Protocol to isolate broadly neutralizing monoclonal antibodies against SARS-CoV-2 from human B cells"

    Article Title: Protocol to isolate broadly neutralizing monoclonal antibodies against SARS-CoV-2 from human B cells

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2025.104275


    Figure Legend Snippet: RT mix-II

    Techniques Used:


    Figure Legend Snippet:

    Techniques Used: Virus, Recombinant, Sterility, Saline, Magnetic Beads, Expressing, Reverse Transcription, Cloning, Luciferase, Plasmid Preparation, Purification, DNA Purification, Software, Cell Culture, Binding Assay, Enzyme-linked Immunosorbent Assay, Adhesive



    Similar Products

    5× first strand buffer  (Thermo Fisher)
    96
    Thermo Fisher 5× first strand buffer
    RT mix-II
    5× First Strand Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5× first strand buffer/product/Thermo Fisher
    Average 96 stars, based on 1 article reviews
    5× first strand buffer - by Bioz Stars, 2026-05
    96/100 stars
    		  Buy from Supplier
    first strand buffer  (Thermo Fisher)
    96
    Thermo Fisher first strand buffer
    RT mix-II
    First Strand Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand buffer/product/Thermo Fisher
    Average 96 stars, based on 1 article reviews
    first strand buffer - by Bioz Stars, 2026-05
    96/100 stars
    		  Buy from Supplier
    strand buffer  (Thermo Fisher)
    96
    Thermo Fisher strand buffer
    a Schematic illustration of the SCAN-seq method. Briefly, control and Hnrnpm cKO GV oocytes were lysed to release <t>mRNAs.</t> <t>First-strand</t> cDNAs were synthesized using a 24-nt barcode RT primer. Following amplification, the cDNAs derived from multiple cells were combined, pooled together, and purified. Subsequently, the full-length cDNAs were used for the construction of nanopore libraries and sequencing. The mouse, oocyte, and nucleic acid schematics are modified adaptations of original resources from SciDraw and Servier Medical Art, respectively, and are employed under a CC BY 4.0 license. b – j PCR and Sanger sequencing validation of genes ( Nlrp14, Meikin, Vrk1 , and Zar1l ) with novel isoforms identified in GV oocytes. Ref. Reference. Representative results shown in ( b – e ) were obtained from at least three independent experiments with similar results. Source data ( b – e ) are provided as a Source data file.
    Strand Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/strand buffer/product/Thermo Fisher
    Average 96 stars, based on 1 article reviews
    strand buffer - by Bioz Stars, 2026-05
    96/100 stars
    		  Buy from Supplier
    1x first strand buffer  (Thermo Fisher)
    99
    Thermo Fisher 1x first strand buffer
    a Schematic illustration of the SCAN-seq method. Briefly, control and Hnrnpm cKO GV oocytes were lysed to release <t>mRNAs.</t> <t>First-strand</t> cDNAs were synthesized using a 24-nt barcode RT primer. Following amplification, the cDNAs derived from multiple cells were combined, pooled together, and purified. Subsequently, the full-length cDNAs were used for the construction of nanopore libraries and sequencing. The mouse, oocyte, and nucleic acid schematics are modified adaptations of original resources from SciDraw and Servier Medical Art, respectively, and are employed under a CC BY 4.0 license. b – j PCR and Sanger sequencing validation of genes ( Nlrp14, Meikin, Vrk1 , and Zar1l ) with novel isoforms identified in GV oocytes. Ref. Reference. Representative results shown in ( b – e ) were obtained from at least three independent experiments with similar results. Source data ( b – e ) are provided as a Source data file.
    1x First Strand Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x first strand buffer/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    1x first strand buffer - by Bioz Stars, 2026-05
    99/100 stars
    		  Buy from Supplier
    5x first strand buffer  (Thermo Fisher)
    99
    Thermo Fisher 5x first strand buffer
    a Schematic illustration of the SCAN-seq method. Briefly, control and Hnrnpm cKO GV oocytes were lysed to release <t>mRNAs.</t> <t>First-strand</t> cDNAs were synthesized using a 24-nt barcode RT primer. Following amplification, the cDNAs derived from multiple cells were combined, pooled together, and purified. Subsequently, the full-length cDNAs were used for the construction of nanopore libraries and sequencing. The mouse, oocyte, and nucleic acid schematics are modified adaptations of original resources from SciDraw and Servier Medical Art, respectively, and are employed under a CC BY 4.0 license. b – j PCR and Sanger sequencing validation of genes ( Nlrp14, Meikin, Vrk1 , and Zar1l ) with novel isoforms identified in GV oocytes. Ref. Reference. Representative results shown in ( b – e ) were obtained from at least three independent experiments with similar results. Source data ( b – e ) are provided as a Source data file.
    5x First Strand Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5x first strand buffer/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    5x first strand buffer - by Bioz Stars, 2026-05
    99/100 stars
    		  Buy from Supplier
    first strand buffer 5x  (Thermo Fisher)
    96
    Thermo Fisher first strand buffer 5x
    a Schematic illustration of the SCAN-seq method. Briefly, control and Hnrnpm cKO GV oocytes were lysed to release <t>mRNAs.</t> <t>First-strand</t> cDNAs were synthesized using a 24-nt barcode RT primer. Following amplification, the cDNAs derived from multiple cells were combined, pooled together, and purified. Subsequently, the full-length cDNAs were used for the construction of nanopore libraries and sequencing. The mouse, oocyte, and nucleic acid schematics are modified adaptations of original resources from SciDraw and Servier Medical Art, respectively, and are employed under a CC BY 4.0 license. b – j PCR and Sanger sequencing validation of genes ( Nlrp14, Meikin, Vrk1 , and Zar1l ) with novel isoforms identified in GV oocytes. Ref. Reference. Representative results shown in ( b – e ) were obtained from at least three independent experiments with similar results. Source data ( b – e ) are provided as a Source data file.
    First Strand Buffer 5x, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand buffer 5x/product/Thermo Fisher
    Average 96 stars, based on 1 article reviews
    first strand buffer 5x - by Bioz Stars, 2026-05
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Journal: STAR Protocols

    Article Title: Protocol to isolate broadly neutralizing monoclonal antibodies against SARS-CoV-2 from human B cells

    doi: 10.1016/j.xpro.2025.104275

    Figure Lengend Snippet: RT mix-II

    Article Snippet: 5× First-Strand Buffer (part of the SuperScript III Reverse Transcriptase) , Thermo Fisher Scientific , Cat#18080044.

    Techniques:

    a Schematic illustration of the SCAN-seq method. Briefly, control and Hnrnpm cKO GV oocytes were lysed to release mRNAs. First-strand cDNAs were synthesized using a 24-nt barcode RT primer. Following amplification, the cDNAs derived from multiple cells were combined, pooled together, and purified. Subsequently, the full-length cDNAs were used for the construction of nanopore libraries and sequencing. The mouse, oocyte, and nucleic acid schematics are modified adaptations of original resources from SciDraw and Servier Medical Art, respectively, and are employed under a CC BY 4.0 license. b – j PCR and Sanger sequencing validation of genes ( Nlrp14, Meikin, Vrk1 , and Zar1l ) with novel isoforms identified in GV oocytes. Ref. Reference. Representative results shown in ( b – e ) were obtained from at least three independent experiments with similar results. Source data ( b – e ) are provided as a Source data file.

    Journal: Nature Communications

    Article Title: hnRNPM cooperates with BCAS2 to modulate alternative splicing during oocyte development

    doi: 10.1038/s41467-026-69176-8

    Figure Lengend Snippet: a Schematic illustration of the SCAN-seq method. Briefly, control and Hnrnpm cKO GV oocytes were lysed to release mRNAs. First-strand cDNAs were synthesized using a 24-nt barcode RT primer. Following amplification, the cDNAs derived from multiple cells were combined, pooled together, and purified. Subsequently, the full-length cDNAs were used for the construction of nanopore libraries and sequencing. The mouse, oocyte, and nucleic acid schematics are modified adaptations of original resources from SciDraw and Servier Medical Art, respectively, and are employed under a CC BY 4.0 license. b – j PCR and Sanger sequencing validation of genes ( Nlrp14, Meikin, Vrk1 , and Zar1l ) with novel isoforms identified in GV oocytes. Ref. Reference. Representative results shown in ( b – e ) were obtained from at least three independent experiments with similar results. Source data ( b – e ) are provided as a Source data file.

    Article Snippet: Then, each sample was mixed with 2.83 μL of reverse transcription mixture, comprising SuperScript IV reverse transcriptase (Invitrogen, 18090050), RNase inhibitor (Takara, Cat. 2313A), SuperScript IV first-strand buffer, 1 M betaine (Sigma-Aldrich, B0300-1VL), 100 mM DTT (Invitrogen, 18090050), 50 mM MgCl2 (Sangon, A610328-0500), and template-switching oligo primers.

    Techniques: Control, Synthesized, Amplification, Derivative Assay, Purification, Sequencing, Modification, Biomarker Discovery